Primer PICKR
Finds and evaluates RT-PCR primer pairs for any gene across 40 species, aggregated and scored from millions of PubMed papers.
This is a web-based tool that helps researchers find and evaluate RT-PCR primer pairs for any gene across 40 species, including mammals, plants, and microbes. It solves the problem of designing effective primers by mining and scoring millions of primer pairs from published literature. The tool is aimed at molecular biologists, geneticists, and other life science researchers in academia and industry. It is positioned as an open-source, evidence-based alternative to traditional primer design software, using a scoring algorithm that combines literature evidence and biophysical properties.
Key features
- Search primers by gene and species
- Filter by melting temperature, GC content, length
- Sort by PICKR score, citations, amplicon length
- View primer pairs with citation counts
- Add primers to list for CSV export
- Bulk export for IDT and Sigma
- Report issues with primer pairs
- Open-source and free to use
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